Leica SP5 Point Scanning Confocal Microscope:
Leica TCS SP5 Spectral Confocal
Inverted Leica DMI 6000 CS microscope base with a conventional scanhead
z-galvo stage for z-stacks and 3D-projections
Objectives: 10x dry (0.4na), 20x multi-immersion (0.7na), 40x oil (1.25na) and a 63x oil (1.4na)
Objectives are coverslip corrected for a #1.5 coverslip (approx. 170um)
4 lasers and a total of 7 laser lines provide flexibility for fluorophore excitation
Lasers: a 405 diode, an argon gas that covers laser lines 458,476,488 and 514, and a 561 diode and a 633 HeNe gas single line
Separate workstation for image analysis and post-processing with a copy of the Leica software
Zeiss Axiovert 200M Microscope:
Workhorse microscope capable of multifluorescence imaging
Filter module with filter sets for: Hoecsht/DAPI, FITC/488/GFP and Texas Red/594
Inverted microscope base
Brightfield, phase contrast and DIC optics
Zeiss AxioCam Mrm CCD camera, Zeiss Axiovision software, motorized stage
Objectives: 10x A-Plan (10x/0.25 Ph1); 20x LD Plan-NEOFLUAR (20x/0.4 Ph2 Korr); 20x Plan-NEOFLUAR (20x/0.50 DIC II); 40x EC Plan-NEOFLUAR (40x/1.3 oil infinity/0.17 DIC III); a 40x LD Plan-NEOFLUOR (40x/0.6 Ph2 Korr infinity/0-1.5); and a 63x Plan-APO CHROMAT (63x/1.4 oil DIC III infinity/0.17)
TMC Micro-g antivibration table
In addition, we have several pieces of accessory equipment for available for use:
2 Eppendorf Transfer Man NK2 micromanipulators
Burleigh Piezo
Patch-clamp electrophysiology
Sutter Micropipette Puller (Model P-87)
Narishige micro-forge MF-900
Leica Wild M3Z dissecting microscope
Wild Heerbrugg dissecting microscope
